To test this, a quick oxidase test for cytochrome c oxidase is performed. Susceptibility test Identification Evaluation of results Identity test Staphylococcus coagulase typing immunoserum “SEIKEN” Staphylococcus coagulase typing reagent “SEIKEN” Differential media: Mannitol salt agar (BBL, Nissui Pharma, etc.) Coagulase production test Identification kit API STAPH (bioMerieux).īefore starting a test, one must confirm the culture is of an Enterobacteriaceae. To guarantee a comparability of different samples, follow the instructions of the manufacturer. An identification is only possible with microbiological culture. The test systems are stored in 20 small reaction tubes, which include the substrates. The API 20E/NE fast identification system combines some conventional tests and allows the identification of a limited number of Gram-negativeEnterobacteriaceae or non-Enterobacteriaceae. The API range introduced a standardized, miniaturized version of existing techniques, which up until then were complicated to perform and difficult to read. Presently, the API test system is manufactured by bioMérieux. It was invented in the 1970s in the United States by Pierre Janin of Analytab Products, Inc. ![]() ![]() API 20NE Detection system after 24 hours incubation.
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